KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| C05 | Produced in house (Ekiert et al.)22 | N/A |
| FluA-20 | Produced in house (Bangaru et al.)29 | N/A |
| Ab6649 | Produced in house (Raymond et al.)35 | N/A |
| Goat anti-human IgG, HRP | SouthernBiotech | Cat# 2040–05; RRID:AB_2795644 |
| Goat anti-mouse IgG, HRP | SouthernBiotech | Cat# 1080–05; RRID:AB_2734756 |
| Bacterial and virus strains | ||
| A/New Caledonia/20/1999 R3ΔPB1 virus | Produced in house | N/A |
| Influenza reporter viruses | Produced in house (Creanga et al.)147 | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| AddaVax adjuvant | Cat# vac-adx-10 | |
| TMB | SeraCare | Cat# 5120–0083 |
| Polyethylenimine (PEI) HCl MAX, Linear, Mw 40,000 | Polysciences | Cat# 24765–1 |
| PNGase F | NEB | Cat# P0705 |
| RDE II | VWR | Cat# 10753–482 |
| Deposited data | ||
| Trihead-1heptad-I53_dn5 | EMDataBank | EMD: 42481 |
| Localized reconstruction of Trihead-1heptad-I53_dn5 | PDB, EMDataBank | PDB ID: 8UR5, EMD: 42482 |
| Trihead-2heptad-I53_dn5 | EMDataBank | EMD: 42483 |
| Trihead-6heptad-I53_dn5 | EMDataBank | EMD: 42485 |
| Localized reconstruction of Trihead-6heptad-I53_dn5 | PDB, EMDataBank | PDB ID: 8UR7, EMD:42486 |
| Experimental models: Cell lines | ||
| Expi293F | ThermoFisher | Cat# A14527 |
| MDCK-SIAT-PB1 | Produced in house (Creanga et al.)147 | N/A |
| Experimental models: Organisms/strains | ||
| BALB/c mice | Jackson Laboratory | Cat# 000651 |
| Recombinant DNA | ||
| See Table S1 for amino acid sequences | ||
| Software and algorithms | ||
| Prism 9 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Leginon | (Suloway et al.)132 | N/A |
| CryoSparc | (Punjanietal.)134 | https://cryosparc.com/ |
| UCSF Chimera | (Pettersen et al.)141 | https://www.cgl.ucsf.edu/chimera/ |
| UCSF ChimeraX | (Goddard et al.)145 | https://www.rbvi.ucsf.edu/chimerax/ |
| PyMol | Schrodinger, LLC | https://pymol.org/2/ |
| Unicorn 7.0 | GE Healthcare | http://www.gelifesciences.com/ |
| Relion | (Zivanov et al.)135 | N/A |
| Other | ||
| 300 mesh grids | Ted Pella | Cat# 01843-F |
| UltrAufoil 2/2, 200 mesh grids | Electron Microscopy Sciences | |
| Filter paper | Cytiva | Cat# 1004047 |
| Uranyl formate | SPI Chem | Cat# 02545-AA |
| Superdex 200 Increase SEC column | Cytiva | Cat# 28-9909-44 |
| Superose 6 Increase SEC column | Cytiva | Cat# 29091596 |
| Excel resin | Cytiva | Cat# 17371203 |
| Isoflurane | USP Patterson | Cat# 07-893-1389 |
| EndoSafe LAL Test Cartridges | Charles River Labs | Cat# PTS20005F |
| Lemo21(DE3) | New England BioLabs | Cat# C2528J |
| MAX Efficiency™ DH5α Competent Cells | Sigma-Aldrich | Cat# I6758 |
| Isopropyl-B-D-thiogalactoside (IPTG) | Thermo Fisher | Cat# 18258012 |
| Kanamycin Sulfate | Sigma-Aldrich | Cat# K1876 |
| HisTrap HP | Cytiva | Cat#17-5247-01 |
| Octet Protein A Biosensors | Sartorius | Cat# 18-5010 |
| Turkey Red Blood Cells | Lampire | Cat# # 7209403 |
| Plasmid Plus Maxi Kit | Qiagen | Cat# 12963 |
| Trypsin, TPCK Treated | Thermo Fisher | Cat# 20233 |