Fig. 1.

Effects of TRPA1 antagonists on CS tear gas agent–induced cutaneous inflammation. (A) CS tear gas exposure and treatment paradigm. The right ears of C57BL/6 male mice were exposed to CS (200 mM, 20 μl) and the left ears to DMSO (solvent for CS, 20 μl). At 0.5 and 4 hours after CS exposure, mice were treated with vehicle (0.5% methylcellulose), HC-030031 (HC), or A967079 (A96) intraperitoneally (i.p.). At 4 hours after CS exposure, mice were injected with IRDye 800CW contrast agent intravenously (i.v.), and in vivo imaging was performed at 5.5 hours after CS exposure. At 6.5 hours after CS exposure, mice were euthanized, ear thickness was measured, and ear punch biopsies were collected. (B–F) Ear thickness, ear punch biopsy weights, and proinflammatory cytokine markers in mice exposed to CS tear gas agent that received either vehicle (0.5% methylcellulose) or TRPA1 antagonist (HC or A96). Data were analyzed by one-way ANOVA with Tukey’s post hoc multiple comparison test. Data are presented as mean ± S.E.M., n = 5 per group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ns = nonsignificant.