Fig. 2.

Effect of TRPA1 antagonists on decreasing CS tear gas agent–induced cutaneous vascular leakage. The right ears of C57BL/6 male mice were exposed to CS (200 mM, 20 μl) and the left ears to DMSO (solvent for CS, 20 μl). At 0.5 and 4 hours after CS exposure, mice were treated with vehicle (0.5% methylcellulose), HC-030031 (HC), or A967079 (A96) intraperitoneally (i.p.). At 4 hours after CS exposure, mice were injected with IRDye 800CW contrast agent intravenously (i.v.), and in vivo imaging was performed at 5.5 hours after CS exposure using MousePOD fitted on Li-COR Odyssey CLX. Representative scan profiles of mice showing profound vascular leakage in the control group (A) and decreased vascular leakage in the treated groups (B and C). Photograph showing MousePOD fitted with Li-COR Odyssey CLX (D). Bar graph showing the quantification of IRDye 800CW contrast agent that leaked into the ear subcutaneous tissue (E). Data were analyzed by one-way ANOVA with Tukey’s post hoc multiple comparison test. Data are presented as mean ± S.E.M., n = 3 to 4 per group. *P ≤ 0.05.