Fig. 3.

Effects of TRPA1 antagonists on CS tear gas agent–induced histopathology features. Right ears of C57BL/6 male mice were exposed to CS (200 mM, 20 μl) and left ears to DMSO (solvent for CS, 20 μl). At 0.5 and 4 hours after CS exposure, mice were treated with vehicle (0.5% methylcellulose), HC-030031 (HC), or A967079 (A96) intraperitoneally (i.p.). At 6.5 hours after CS exposure, mice were euthanized and ear punch biopsies were collected for histopathological analysis. Representative H&E-stained histopathologic sections from (A) DMSO (solvent for CS), (B) CS + vehicle, (C) CS + HC030031, and (D) CS + A967079 groups are presented at 20× magnification. Black arrow heads = infiltration of leukocytes. d, dermis; e, epidermis; ec, elastic cartilage; hf, hair follicle.