Fig. 4.

Persistent skin injuries after CS tear gas agent exposure and effects of TRPA1 antagonists on skin inflammation in an extended mouse observation model. (A) CS tear gas exposure and treatment. Right ears of C57BL/6 male mice were exposed to CS (200 mM, 20 μl) and left ears to DMSO (solvent for CS, 20 μl). At 0.5, 4, 24, and 48 hours after CS exposure, mice were treated with vehicle (0.5% methylcellulose), HC-030031 (HC), or A967079 (A96) intraperitoneally (i.p.). (B–E) Ear thickness, ear punch biopsy weights, and cytokine markers in mice receiving vehicle or TRPA1 antagonist after exposure to CS tear gas agent. Data were analyzed by one-way ANOVA with Tukey’s post hoc multiple comparison test. Data are presented as mean ± S.E.M., n = 5 per group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ns = nonsignificant.