Fig. 6.

Effects of advanced TRPA1 antagonists on CS tear gas agent–induced calcium influx. HEK293T cells were transfected with mouse (A and B) and human (C and D) TRPA1 plasmid. (A and C) Show dose-response curve of CS tear gas [mouse: EC₅₀ = 0.47 μM, 95% confidence intervals (CI) = 0.32–0.83 μM; human: EC₅₀ = 0.016 μM, 95% CI = 0.01–0.024 μM]. (B and D) TRPA1 antagonists [A967079 (A96), AMG0902, or GDC0334] inhibited the calcium influx response elicited by CS tear gas in mouse and human TRPA1-transfected cells. IC₅₀ and 95% CI values are presented in tables under Fig. 6, B and D. Data are presented as mean ± S.E.M. Each experiment was performed with a minimum of three replicates.