Fig. 2.
Elf1 enhances interactions between Pol II and Rad26. (A) Deletion of Elf1 leads to UV sensitivity and can be rescued by Elf1core. (B) Elf1, or Elf1core have no effect on the stalling of Pol II at a CPD lesion. Time points were 0.3, 1, 3, and 10 min. (C) Mass photometry of EC + Rad26 in the absence or presence of Elf1. Peak identities and predicted molecular weights are indicated. (D) Kds for the interaction between Pol II (EC) stalled at a CPD lesion and Rad26 in the absence or presence of Elf1 were determined from multiple repeats of the experiment in (C). P < 0.0001, two-tailed Student’s t test. Data are mean and SD (n = 12). The final Elf1 concentrations were 1 μM (B) and 0.5 μM (C). All assays were performed at least three times independently.