Fig. 1.

Digital SAA. (A) A pathological hallmark of PD, Lewy bodies are primarily composed of α-synuclein aggregates, which can be detected with SAAs by combining pathological seeds with wild-type monomers and an amyloid fibril staining dye. By transforming the bulk SAA into a digital format by partitioning the reaction into microcompartments, the number of single aggregate molecules can be quantified. (B) The three compartmentalization strategies used to develop digital SAA platforms, included microwell arrays, droplets, and alginate hydrogel microcapsules.