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. 2024 Jan 11;121(3):e2220532121. doi: 10.1073/pnas.2220532121

Fig. 2.

Fig. 2.

Inducible Dicer deletion modifies EEG slow-wave (SW) properties following spontaneous and enforced (SD) waking. (A) From Top to Bottom: Mean time course of NREMS EEG delta power (δ; also see Fig. 1C) and NREMS and REMS hourly amounts, during 24 h baseline (BL) and 18 h of recovery (REC) from a 6 h SD (black rectangles) starting at light onset (ZT0). Gray areas delineate the dark periods. Significant genotype changes are depicted by red horizontal lines above the data (two-way rANOVA interaction factors genotype × time P < 0.001; post hoc t tests P < 0.05). (B) NREMS (Left) and REMS (Right) rebound for the 18 h of recovery after SD as accumulated differences from corresponding baseline hours. Significant differences are marked by red horizontal lines underneath the data (t tests P < 0.05). (C) Average spectral profiles for wake (W), NREMS (N), and REMS (R), during 48 h of baseline expressed in absolute values (μV2/0.25 Hz). Differences between group means are displayed below in black. Significant genotype changes are depicted by red horizontal lines below the data (two-way rANOVA interaction factors genotype × time P < 0.001; post hoc t tests P < 0.05). (D) From Top to Bottom: SW density and amplitude at different times of the experiment during which sleep need is assumed to differ (also see description for Fig. 1E). Frequencies with significant changes in SW density or amplitude compared to controls (gray) are depicted by the red squares (two-way rANOVA interaction factors genotype × frequency; P < 0.001 for all periods; post hoc Tukey: P < 0.05). (E) Time-course of δ1 (0.75 to 1.75 Hz; Top) and δ2 (2.5 to 3.5 Hz; Bottom) spectral power during baseline and following 6 h SD (black rectangles), in icKO mice and controls. Statistics (two-way rANOVA interaction genotype × time; δ1: P < 0.001; δ2: P < 0.05). All values represent means (solid lines) ± SEM (shaded areas). Power was expressed as % of mean δ1 and δ2 power, respectively, during the last 4 h of the light period of baseline (ZT8-12). N = Dicerfl/fl + vehicle: 6; Dicerfl/fl + tamoxifen: 7.