TABLE 2.

Phenotype and response to B. burgdorferi of synovial fluid lymphocytes from Lyme arthritis patients^a

Patient no.	% of cells positive for:		Proliferation (cpm) withb:
	CD4	CD8	Medium only	Medium plus B. burgdorferi sonicate
1	26.5	33.7	7,132	40,055
2	41.0	32.0	6,442	11,127
3	19.3	9.1	8,136	106,711
4	52.6	28.2	526	19,122
5	27.8	45.7	17,369	82,440
6	24.7	41.3	4,516	51,278
7	33.6	24.4	2,230	4,463
8	45.4	35.3	30,230	89,533
9	29.7	45.3	5,316	178,964

^aSynovial fluid lymphocytes were isolated with Ficoll-Hypaque. Freshly isolated specimens were stained for CD4 and CD8 and analyzed by flow cytometry. Samples (5 × 10⁴ cells/well) were also cultured in serum-free medium in the absence or presence of a sonicate of B. burgdorferi (3 μg/ml). Proliferation was measured after 7 days by measuring [³H]thymidine incorporation over the last 18 h. 

^bValues are the means of data from triplicate cultures. Standard deviations were less than 15%.