Fig. 4.

Effect of IDO1 on IFN-γ-mediated macrophage M2 polarization. A The transfection efficiency was verified by Western blot; B The expression of CD163 and CD206 was detected by Western blot; C The levels of IL-6, IL-10, IL-13 and TNF-α were measured by ELISA; D The phagocytic function of TAMs was observed by cell probe staining. E The phagocytosis of TAMs was detected by flow cytometry. Compared with si-NC group or NC group,^* P < 0.05,^** P < 0.01,^*** P < 0.001; Compared with IL-4 group,^# P < 0.05,^## P < 0.01,^### P < 0.001; Compared with IL-4 + IFN-γ group,^$ P < 0.05,^$$ P < 0.01 and ^$$$ P < 0.001