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Purified Tf190 contains carbohydrate structures. (A) HPLC chromatogram of affinity-purified Tf190. Peaks: 1, fucose; 2, galactosamine; 3, glucosamine; 4, galactose; 5, glucose; 6, mannose. (B) Silver staining pattern of affinity-purified Tf190 after SDS-PAGE. Tf190 was electrophoresed on a 10% T gel. PAD, pulse amperometric detector. STD, sugar standards.
