TABLE 2.
Expression of Tf190 on the surfaces of live T. foetus cells as determined by flow cytometry
| Antibody | Intensityb | Mode fluorescence (%)a for strain:
|
||||
|---|---|---|---|---|---|---|
| 330.1 | Y-32 | BQM | 17905 | BP-4 | ||
| 32.3B3.5c,d | − | 9 (64) | 5 (71) | 20 (1) | NDf | ND |
| + | 22 (28) | 35.7 (29) | 5 (98) | 29 (99) | 101 (100) | |
| 32.3B3.3d | − | 6.5 (76) | 5.7 (69) | 20 (2) | ||
| + | 20 (24) | 29.8 (31) | 5.3 (98) | ND | ND | |
| 32.1B4.2d | − | 23 (2) | 26 (2) | 21 (1) | ||
| + | 4.7 (98) | 4.1 (98) | 5.3 (98) | ND | ND | |
| 32.8D3.2d | − | 5.7 (64) | 10 (61) | 7 (84) | ||
| + | 33 (35) | 23 (39) | 21.5 (16) | ND | ND | |
| Rabbit anti-Tf190e | − | 19 (42) | 19 (39) | 13 (40) | ||
| + | 32 (58) | 42.8 (60) | 37 (47) | ND | ND | |
a
Results are values of mode fluorescence (with percentages of gated events in parentheses) based on 10,000 events. The mean value of mode fluorescence ± the standard deviation for controls without primary antibody was 4.03 ± 1.2 (range, 2.8 to 5.5).
b
+, fluorescence intensity above 1.9 × 101; −, fluorescence intensity below 1.9 × 101.
c
MAb originally defining Tf190 and used to affinity purify the molecule.
d
MAbs reacting with affinity-purified Tf190 by DIA.
e
Rabbit antiserum prepared against affinity-purified Tf190.
f
ND, not determined.