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. 2023 Nov 18;44(6):1003–1014. doi: 10.24272/j.issn.2095-8137.2023.108

Figure 2.

Figure 2

Diabetic mice show more severe symptoms after SARS-CoV-2 infection

A: Schematic of hACE2-Lepr-/- mouse model construction and challenge procedures. B, C: Body weight changes (B) and survival rates (C) of hACE2-Lepr-/- and hACE2-Lepr+/+ mice after SARS-CoV-2 infection. D: Blood glucose levels in hACE2-Lepr-/- and hACE2-Lepr+/+ mice before and after infection. E: SARS-CoV-2 N, ORF1ab, and S gene levels in lungs of hACE2-Lepr-/- and hACE2-Lepr+/+ mice were detected by qPCR. F: IHC assay of SARS-CoV-2 N protein in lungs of hACE2-Lepr-/- and hACE2-Lepr+/+ mice after virus infection. 5×, scale bar represents 400 µm; 20×, scale bar represents 100 µm. G: Histopathological changes in lung tissues of hACE2-Lepr-/- and hACE2-Lepr+/+ mice after virus infection (arrow indicates inflammatory cell infiltration, bold arrow indicates hyaline membrane). 5×, scale bar represents 200 µm; 20×, scale bar represents 50 µm. H: Lung pathological (H&E) scores (left) were counted according to INHAND standard rules, and SARS-CoV-2 N protein-positive area (right) was calculated using Case Viewer. I: IHC assay for HIF-1α in lungs of hACE2-Lepr-/- and hACE2-Lepr+/+ mice after infection. J: TUNEL immunofluorescence staining of lungs in hACE2-Lepr-/- and hACE2-Lepr+/+ mice. I, J: Shown at 5×, scale bar represents 300 µm; 20×, scale bar represents 100 µm.