FIG. 2.

PD98059 does not kill intracellular Listeria or Salmonella. HeLa cells were infected with L. monocytogenes 1/2a₃ for 60 min or S. typhimurium SL1344 for 30 min. Various concentrations of PD98059 were then added along with 100 μg of gentamicin per ml in MEM for 120 min. Appropriate amounts of the solvent DMSO were also added so that all wells contained equivalent concentrations of DMSO. The monolayers were lysed, and the numbers of intracellular bacteria were determined by plating. Experiments were repeated at least three times with similar results, and the graph depicts one representative experiment. Each data point is the mean of data from three wells, and the standard deviation is shown by the error bar.