FIG. 6.
Various inhibitors reduce L. monocytogenes invasion. HeLa cells were pretreated with 1 μg of cytochalasin D per ml, 100 nM wortmannin, or 250 μM genistein for 30 min prior to bacterial infection. Control cells were treated with 0.2% DMSO in MEM. L. monocytogenes 1/2a3 was allowed to invade for 60 min before the addition of 100 μg of gentamicin per ml. The monolayers were lysed, and the numbers of intracellular bacteria were determined by plating. The assay was repeated at least three times with similar results, and the graph depicts one representative experiment. Each data point is the mean of data from three wells, and the standard deviation is shown by the error bar.