Abstract
The pharmaceutical interest and limited availability of γ-linolenic acid (GLA) and eicosapentaenoic acid (EPA) prompted the search for genetic means for increasing the production of these fatty acids from algal sources. Cell lines of Spirulina platensis and Porphyridium cruentum resistant to the growth inhibition of the herbicide Sandoz 9785 were selected by serial transfers of the culture in the presence of increasing concentrations of the herbicide. The resistant cell lines of S. platensis overproduced GLA and those of P. cruentum overproduced EPA and were stable for at least 50 generations in the absence of the inhibitor.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Harris W. S. Fish oils and plasma lipid and lipoprotein metabolism in humans: a critical review. J Lipid Res. 1989 Jun;30(6):785–807. [PubMed] [Google Scholar]
- Riccardi G., Sora S., Ciferri O. Production of amino acids by analog-resistant mutants of the cyanobacterium Spirulina platensis. J Bacteriol. 1981 Sep;147(3):1002–1007. doi: 10.1128/jb.147.3.1002-1007.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walsh C. T. Suicide substrates, mechanism-based enzyme inactivators: recent developments. Annu Rev Biochem. 1984;53:493–535. doi: 10.1146/annurev.bi.53.070184.002425. [DOI] [PubMed] [Google Scholar]
- Willemot C., Slack C. R., Browse J., Roughan P. G. Effect of BASF 13-338, a Substituted Pyridazinone, on Lipid Metabolism in Leaf Tissue of Spinach, Pea, Linseed, and Wheat. Plant Physiol. 1982 Jul;70(1):78–81. doi: 10.1104/pp.70.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright S., Burton J. L. Oral evening-primrose-seed oil improves atopic eczema. Lancet. 1982 Nov 20;2(8308):1120–1122. doi: 10.1016/s0140-6736(82)92784-2. [DOI] [PubMed] [Google Scholar]