Figure 3. Lens mRNA levels of each α-crystallin gene in larvae (10 and 19 day), juveniles (5 week) and adults.

Box and whisker plots are shown for three RT-qPCR biological replicates. The Y-axes are inverted as they show delta Cq values that account for two endogenous control genes (rpl13a and eef1a1l1), with lower values indicating higher gene expression. Data used for this analysis are included as Supplemental Table 4.