Fig. 5: ScoC, PchR, and AbrB Bind Near the Core Promoter Region of yvmC.
Electrophoretic mobility shift assays with WT PyvmC (A) and the Δ59 promoter PyvmCΔ59 (B) with increasing concentrations of purified PchR (left panel), AbrB (middle panel), and ScoC (right panel). Unshifted bands are marked with unfilled triangles, shifted bands are marked with filled triangles, and smears are marked with brackets. Proteins were diluted two-fold and final concentrations are as follows: PchR (15.6 to 250 nM for WT, 125–250 nM for Δ59), AbrB (62.5 to 1000 nM for WT, 500–1000 nM for Δ59), and ScoC (125 to 1000 nM for WT, 250–1000 nM for Δ59).