Table 2.
Amino acid polymorphisms in the HA protein for H7 viruses passaged in MDCK-wt and MDCK-Gc cells.
| Virus | ||||||
|---|---|---|---|---|---|---|
| rgCk/WX13 | rgMall/NJ10 | |||||
| Position (H3 numbering) | SEED | MDCK-WT (P5) | MDCK-GC (P5) | SEED | MDCK-WT (P5) | MDCK-GC (P5) |
| 88 (98; RBS) | Y | Y | Y | Y | Y | Y |
| 124 (134; RBS) | G | G | G | G | G | G |
| 125 (135; RBS) | A |
T (87.39) A (12.20) |
A (51.49) T (24.91) |
A | A | A |
| 126 (136; RBS) | T | T | T | T | T | T |
| 127 (137; RBS) | S | S | S | S | S | S |
| 128 (138; RBS) | A | A | A | A | A | A |
| 133 (144) | G | G | G | G |
G (63.80) D (35.70) |
G (96.37) D (3.07) |
| 142 (153; RBS) | W | W | W | W | W | W |
| 151 (160; RBS) |
A (87.39) T (12.20) |
A (70.75) T (29.01) |
A (76.63) T(23.15) |
A |
A (93.24) D (5.63) |
T (78.75) A (20.73) |
| 174 (183; RBS) | H | H | H | H | H | H |
| 179 (188; RBS) | T | T | T | A | A | A |
| 180 (189; RBS) | A | A | A | T | T | T |
| 181 (190; RBS) | E | E | E | E | E | E |
| 182 (191; RBS) | Q | Q | Q | Q | Q | Q |
| 183 (192; RBS) | T | T | T | T | T | T |
| 184 (193; RBS) | K | K | K | K |
K (82.68) T (16.44) |
K (87.62) T (7.81) |
| 185 (194; RBS) | L | L | L | L | L | L |
| 186 (195; RBS) | Y | Y | Y | Y | Y | Y |
| 210 (219) | A |
A (83.65) E (15.30) |
A (84.22) E (15.61) |
A | A | A |
| 212 (221; RBS) | P | P | P | P | P | P |
| 213 (222; RBS) | Q | Q | Q | Q | Q | Q |
| 214 (223; RBS) | V | V | V | V | V | V |
| 215 (224; RBS) | D | N | N | N | N | N |
| 216 (225; RBS) | G | G | G | G |
G (68.94) E(30.11) |
G (93.96) E (5.55) |
| 217 (226; RBS) | L | L | L | Q | Q | Q |
| 218 (227; RBS) | S | S | S | S | S | S |
| 219 (228; RBS) | G | G | G | G | G | G |
| 241 (250) | A | A |
A (81.98) T (17.66) |
A | A | A |
| 322 (330) |
G (96.40) V (3.28) |
G |
G (84.71) V(14.65) |
G | G | G |
| 453 (461) | E | E |
E (82.94) G (7.74) |
E | E | E |
| 454 (462) | D | D |
D (89.99) I (7.77) |
D | D |
D (95.95) F (2.91) |
| 455 (463) | G | G |
G (88.88) R (10.74) |
G | G | G |
| 456 (464) | T | T |
T (85.30) S(13.96) |
T | T |
T (96.50) I (13.32) |
| 457 (465) | G | G |
G (81.79) D (17.47) |
G | G |
G (91.75) V (5.61) |
| 458 (466) | C | C | C | C | C |
C (97.34) S (1.29) |
| 459 (467) | F | F |
F (97.86) I (8.46) |
F | F |
F (93.55) L (2.94) |
| 460 (468) | E | E (79.86) Q (18.45) |
E | E | E |
E (96.55) R (2.78) |
| 461 (469) | I | I (79.64) K (19.59) |
I | I | I |
I (84.64) R (13.29) |
| 462 (470) | F | F (78.60) C (20.77) |
F | F | F |
F (83.29) Q (14.26) |
| 463 (471) | H | H | H | H | H | H (82.98) N (15.87) |
The positions with < 98% predominance for a single amino acid were listed in parenthesis, and the predominant amino acids are highlighted in bold. RBS, receptor binding sites.