Figure 2. ϕBB-1 phage titer, virion morphology, and proteomic analysis.

(A) Packaged, DNase-protected cp32 copy numbers in bacterial supernatants were measured by qPCR. Data are the SE of the mean of three experiments, ***p <0.001. (B) Virions were purified from 4-L cultures of B. burgdorferi CA-11.2A and imaged by transmission electron microscopy. Representative images from two independent preparations are shown. (C and D) HPLC-MS/MS-based proteomics was used to identify proteins in two purified virion preparations. The SE of the mean of spectral counts for peptides associated with the indicated phage structural proteins are shown for each replicate. See also Table S1 for the complete proteomics dataset.