Fig 6. cp32s are linearized upstream of the erp loci.

(A) PhageTerm was used to predict the linear ends of packaged cp32 molecules. (B–F) Nanopore reads were mapped to the indicated cp32s. Note the sharp boundary just upstream of the erp loci (highlighted in red). The yellow triangles indicate the PhageTerm predicted linear ends. (G) Alignments of the intergenic region upstream of the erp loci is shown for each cp32. Colors indicating A, T, C, or G are shown in panel H. The black line indicates the pac region that was cloned into a shuttle vector, as described in Figure 7. (H) A nucleic acid logo was constructed from 207 cp32 sequence alignments. Yellow triangles indicate the linear end of cp32 isoforms as predicted by PhageTerm and confirmed by long-read sequencing.