Figure 2: 3,5 difluorophenol Substituted Long Chain Phosphonate is a potent inhibitor of parasite growth.

(A) Strucutre of Salinipostin A and constitutional isomers of long chain mixed alkyl/aryl phosphonates.

(B) IC₅₀ curves of short chain mixed alkyl/aryl phosphonates for inhibition of 4-methylumbelliferyl caprylate processing by recombinant PfMAGL. (mean ± SD, N,n = 2,3).

(C) EC50 values of Compound 21 and 22 in 72 in a 72 hour treatment of synchronized ring stage W2 parasites with error bars, SEM. (mean ± SD, n=4 from 3 independent experiments).

(D) Rate of Kill of Compound 22 measured against compared against known fast (chloroquine), medium (pyrimethamine), and slow (atovaquone) acting inhibitors of parasite growth in which compounds were dosed at their EC₅₀ and parasite viability was monitored over time and ± SEM. N,n = 6,2.

(E) HFF Toxicity for alkyl cyclic peptide inhibitor and linear counterparts. Points are plotted as mean ± SEM values of each inhibitor for HFF growth. Data were generated with 72 hour assays (N, n = 2,3).