Figure 5: Compound 22 has a mechanism of action different from the pan lipase inhibitor Orlistat.

(A) Giemsa-stained parasites. Synchronized ring-stage parasites were treated at 0–6 hours post-invasion with DMSO (vehicle control), 10 μM compound 21 or 22, and imaged at 24 h, 36 h, and 48 h.

(B) Lipidomic analysis of compound 22 treated parasites. Synchronized ring stage parasites were treated with either 900 nM compound 22 or DMSO for 24 hours before lysis and collection of lipids. Significantly changed lipid classes are denoted on volcano plot. N= 3–4

(C) Isobolograms depicting P. falciparum in vitro culture-based fractional EC₅₀ (FIC50) values in the presence of compound 22 and Orlistat, each point represents a different biological replicate done in technical duplicate.