Figure 1: Mice with experimental DVT exhibit increased plasma G-CSF levels, neutrophilia, and neutrophil hyperactivation.

(A) Schematic of experimental design. (B) Plasma G-CSF levels in mice with IVC stenosis and with sham-surgery at indicated time points. Blood was collected 3-hr post-surgery from mice with sham-surgery and mice with IVC stenosis for analysis of (C) Blood neutrophil/lymphocyte ratio, (D) cell-free DNA, (E) plasma elastase, and (F) plasma MPO. All data are from male mice and are mean ± SEM and analyzed by 2-way repeated measures ANOVA (Kruskal-Wallis test) followed by Fisher’s LSD test (B), or an unpaired Student t test (C-F); n = 3 (B) and n = 6 (C-F).