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[Preprint]. 2024 Jan 3:rs.3.rs-3810214. [Version 1] doi: 10.21203/rs.3.rs-3810214/v1

Figure 1. Neurogranin (Nrgn) is dysregulated in PWH at the mRNA and protein levels.

Figure 1

Representative images of postmortem frontal cortex (FC) neurons stained for Nrgn (Cy3, red) and DAPI (blue) in (A) control and (B) people with HIV-1. The images are z-projections of image stacks acquired at ×60 magnification; scale bar is 10 mm. (C) Quantification of Nrgn levels in whole frontal cortex lysates from people with HIV-1 (N=13) in comparison with control individuals (N=15). (D) Relative expression of Nrgn mRNA in FC brain samples from HIV-1 positive individuals (N=49) compared to control individuals (N=22) as assessed by RT-qPCR. (E) Quantification of Nrgn mRNA copy number in FC samples of HIV-1 positive individuals compared to control individuals. (F) A standard curve obtained from serial dilutions of full-length Nrgn construct as template for the qPCR reaction to calculate copy number.