FIG. 1.

Map of B. afzelii IP21 cp8.3 indicating the location of ORFs, IR elements, and oligonucleotide binding sites. The sequence of cp8.3 was determined by Dunn et al. (13). The direction of possible transcription of each ORF is indicated by arrows. Oligonucleotides with designations including the letter R (reverse) that are not shown in the figure target the identical sites as their forward (F) primer counterparts except that they bind to the opposite strand. The map was generated with the MacPlasmap (Jindong Liu, University of Utah) and Canvas (Deneba) programs.