FIG. 2.

RFLP pattern analysis of ORF 2 among B. burgdorferi sensu lato isolates. DNA from each isolate was digested to completion with EcoRV, fractionated, blotted, and probed with an ORF 2 PCR-generated probe as discussed above. Isolates analyzed were B. burgdorferi LP7, CA9, and 297 (lanes 1 to 3, respectively); B. garinii FRG and N34 (lanes 4 and 5, respectively); B. valaisiana VS116 (lane 6); B. garinii VSBP (lane 7); B. afzelii IP21, ECM1, and UMO1 (lanes 8 to 10, respectively); B. andersonii 19857 and 21038 (lanes 11 and 12, respectively); and B. japonica IKA2 and HO14 (lanes 13 and 14, respectively). Molecular size standards (in kilobases) are indicated on the left.