FIG. 6.

PCR and sequence analyses of IRA and its flanking regions. (A) Amplicons obtained with the O7R1 and O1R1 primers are presented. The O7R1-O1R1 primer pair was designed to amplify across IRA of cp8.3. For lane identification, see the legend to Fig. 2. (B) Selected amplicons were partially sequenced. Gaps introduced by alignment are indicated by dashes, conserved nucleotides are indicated by periods, and the sequence corresponding to IRA is indicated by boldface letters. The start codon of ORF 8 is underlined and indicated with italic letters.