TABLE 1.
B. burgdorferi sensu lato complex isolates investigated
| Isolate | Origina and location | No. of passages | Summary of PCR resultsb
|
spPCR results | |||
|---|---|---|---|---|---|---|---|
| rrs | ORF 1 | ORF 2 | ORF 4 | ||||
| B. burgdorferi | |||||||
| LP7 | Human EM; Connecticut | 3 | + | − | − | + | − |
| CA9 | Ixodes pacificus tick; California | 3 | + | − | − | + | + |
| 297 | Human CSF; Connecticut | ?c | + | − | − | ± | − |
| B. garinii | |||||||
| FRG | Ixodes ricinus tick; Germany | ? | + | − | − | + | − |
| N34 | Ixodes ricinus tick; Germany | ? | + | − | − | + | + |
| VSBP | Ixodes ricinus tick; Switzerland | 9 | + | − | − | ± | − |
| B. valaisiana sp. nov. VS116 | Ixodes ricinus tick; Switzerland | 10 | + | − | − | − | − |
| B. afzelii | |||||||
| IP21 | Ixodes persulcatus tick; Russia | >20 | + | + | + | + | + |
| ECM1 | Human EM; Sweden | ? | + | − | +d | + | − |
| UMO1 | Human EM; Sweden | ? | + | + | + | + | + |
| B. andersonii | |||||||
| 19857 | Sylvilagus floridans (rabbit) kidney; New York | 7 | + | − | − | + | − |
| 21038 | Ixodes dentatus tick; New York | 6 | + | − | − | ± | − |
| B. japonica | |||||||
| IKA2 | Ixodes ovatus tick; Japan | ? | + | − | − | ± | − |
| HO14 | Ixodes ovatus tick; Japan | ? | + | − | − | + | − |
a
Abbreviations: EM, erythema migrans; CSF, cerebrospinal fluid.
b
+, positive; −, negative; ±, weak product (relative to that of the other isolates).
c
?, unknown.
d
The amplicon obtained from this isolate was approximately 300 bp shorter than predicted.