TABLE 2.

Summary of Southern hybridization results

Species and isolate	Hybridization results for target sequencea
	ORF 1					ORF 2					ORF 4					IR element
	O1F1 oligo	O1R2 oligo	PCR probe (IP21)b	PCR probe (2D CHEF)		O2F1 oligo	O2R2 oligo	PCR probe (IP21)b	PCR probe (2D CHEF)		O4R3 oligo	PCR probe (IP21)b	PCR probe (CA9)b	PCR probe (2D CHEF)		IR-AF1 oligo	2D CHEF (IR-AF1 oligo)
				LP	CP				LP	CP				LP	CP		LP	CP
B. burgdorferi
LP7	−	−	−	ND	ND	−	−	+M	ND	ND	+	+M	+M	ND	ND	+M	ND	ND
CA9	−	−	+	ND	ND	−	−	+M	ND	ND	+M	+M	+M	ND	ND	+M	ND	ND
297	−	−	+M	ND	ND	−	−	±	ND	ND	+M	+M	+M	ND	ND	−	ND	ND
B. garinii
FRG	−	−	+M	+M	+	−	−	+M	+	+M	+M	+M	+M	+M	+M	+M	+M	+M
N34	−	−	+M	ND	ND	−	−	−	ND	ND	+M	+M	+M	ND	ND	−	ND	ND
VSBP	−	−	+M	ND	ND	−	−	−	ND	ND	+M	+M	±	ND	ND	−	ND	ND
B. valaisiana VS116	−	−	−	±	−	±M	−	+M	−	+M	+	+M	+M	−	±	+M	+M	+M
B. afzelii
IP21	+	+	+	+M	+	+	+	+	+	+M	+	+M	+	+	+M	+	−	−
ECM1	+	+	+	ND	ND	+	+	−	ND	ND	+M	+M	+M	ND	ND	−	ND	ND
UMO1	+	+	+	ND	ND	+	+	+M	ND	ND	+M	+M	+M	ND	ND	+M	ND	ND
B. andersonii
19857	−	−	−	ND	ND	−	−	−	ND	ND	−	+M	−	ND	ND	−	ND	ND
21038	−	−	−	ND	ND	−	−	−	ND	ND	−	+M	−	ND	ND	−	ND	ND
B. japonica
IKA2	−	−	−	ND	ND	−	−	−	ND	ND	−	−	−	ND	ND	−	ND	ND
HO14	−	−	−	ND	ND	−	−	−	ND	ND	−	+M	+M	ND	ND	−	ND	ND

^aWith the exception of the Southern hybridizations involving 2D CHEF gels (as indicated at the top of the appropriate columns), all other hybridizations were conducted with EcoRV-digested DNA. 2D CHEF hybridization results are divided into two columns to indicate whether LPs or CPs hybridized with the probes. Hybridization results are scored as either +, ±, or −, for strong, weak, or no hybridization, respectively. Superscript M indicates that multiple hybridizing fragments or plasmids were detected. ND, not done; oligo, oligonucleotide. 

^bThe isolate from which the probe was generated is indicated in parentheses.