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. Author manuscript; available in PMC: 2024 Jan 22.
Published in final edited form as: Dev Biol. 2021 May 24;477:177–190. doi: 10.1016/j.ydbio.2021.04.010

Figure 5. Shha+ basal epidermis and pre-osteoblasts are intertwined in developing fins.

Figure 5.

(A-F) Fluorescence widefield (A-C) or confocal (D-F) images of the dorsal caudal fin lobe of a 28 dpf shha:GFP;runx2:mCherry fish. (A-C) shha:GFP-expressing basal epidermal cells (green) overlay and extend distally from runx:mCherry-high pre-osteoblast (magenta). The overlay in (C) includes a brightfield image for context. (D-F) A single optical slice (E; sagittal; orientation key in E’) and reconstructed longitudinal (D) and transverse (F) views of a distal ray region undergoing ray branching. shha:GFP+ basal epidermis (green) and runx2:mCherry+ pre-osteoblasts (magenta) have overlapping signal at interfaces, indicating their tight juxtaposition. Basal epidermis and pre-osteoblasts tandemly separate into split pools during branching (white arrows). The grey dotted oval highlights a ridge of pre-osteoblasts nestled into a shha:GFP+ basal epidermal groove (Movie 2). Scale bars are 250 μm (A-C) and 10 μm (D-F).