Fig 7. Piscidins cause leakage of glycolipid containing membranes.
Fractional leakage of calcein through membrane vesicles comprised of 45 μM suspensions of 2:1 (16:0 18:1) POPG: (16:0–18:1) DG-Glucose is shown as a function of the peptide/lipid ratio at (A) 37°C and (B) 45°C. The assay was duplicated and one representative replicate is shown. Each data point is the average of independent measurements made in triplicate. The EC50 values based on fitting the two duplicates are provided in Table 2.