FIG. 6.

Protection of crp DNA from DNase I by rEUO. (A) Top strand; (B) bottom strand. Protected regions are indicated by vertical lines. DNA sequencing ladders (in base pairs) prepared with the same primers used to generate the probes in the protection assay are shown at the left side of each panel. The double arrow indicates hypersensitive bands at positions −16 and −17. The DNA sequence (with the protected region bracketed) is shown at the bottom of each panel. The −35 and −10 promoter elements are underlined.