FIG. 7.

Comparison of binding of native EUO and rEUO to crp operon promoter DNA. Gel shift assays were carried out with 1 ng of crp probe DNA (bp −200 to +67) as described in Materials and Methods. Lanes: 1, free probe; 2, probe plus 10 ng of rEUO; 3, probe plus crude L-cell extract; 4, probe plus 15-h p.i. RB extract; 5, probe plus 29-h p.i. RB extract; 6, free probe; 7, probe plus rEUO and anti-His tag rEUO; 8, probe plus L-cell extract and anti-rEUO; 9, probe plus 15-h p.i. RB extract and anti-rEUO; 10, probe plus 29-h p.i. RB extract and anti-rEUO.