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. 2024 Jan 22;7(4):e202302177. doi: 10.26508/lsa.202302177

Figure 2. Dmel_CG41128 is DmMIC10b, a bona fide subunit of MICOS in D. melanogaster.

Figure 2.

(A) Western blot analysis of steady-state protein levels in solubilized mitochondria from WT and CG41128[KO] flies. (B) Co-IP from solubilized mitochondria from WT flies. DmMIC60 and CG41128 were used as a bait. (C) Co-IP from whole-cell lysates. Human MIC10-FLAG (HsMIC10) and CG41128-FLAG were transiently expressed in HeLa cells or COS-7 cells. FLAG-tagged proteins were used as a bait. (D, E) Electron micrographs of mitochondria in brain tissue from WT and CG41128[KO] flies. n, number of analyzed mitochondrial cross sections. (D) Representative images of mitochondria. (E) Quantification of the cristae architecture. Scale bars: 0.5 μm.