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. 2024 Jan 22;7(4):e202302177. doi: 10.26508/lsa.202302177

Figure 5. DmMIC10b polymerizes into filaments, which alter the mitochondrial ultrastructure.

Figure 5.

(A) Live-cell STED recordings of mammalian cells expressing DmMIC10b. COS-7 and HeLa cells were co-transfected with DmMIC10b-FLAG and COX8A-SNAP to visualize the crista membranes. Cells were labeled with SNAP-cell SiR-647 and visualized by 2D live-cell STED nanoscopy. (B) Electron tomography of mitochondria from COS-7 cells expressing DmMIC10b-FLAG. The recording shows a cross section (mito-1) and a longitudinal section (mito-2) of two adjacent mitochondria. Membranes (blue) and filaments (yellow) were semi-automatically segmented and are shown as volume renderings. Abbreviations: OM, outer membrane; IBM, inner boundary membranes; CM, crista membrane. Scale bars: 2 μm (A) and 0.25 μm (B).