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. 2024 Jan 22;7(4):e202302177. doi: 10.26508/lsa.202302177

Figure S6. DmMIC10b expression induces filament formation in the absence of human MICOS proteins and irrespective of the C-terminal FLAG-tag.

Figure S6.

(A, B) Genome-edited HeLa cells expressing DmMIC10b-FLAG were immunolabeled against the FLAG epitope and recorded with 2D STED nanoscopy. (A) HeLa cells depleted of HsMIC10 (MIC10-KO), which are lacking the MIC10 subcomplex. (B) HeLa cells depleted of HsMIC60 (MIC60-KO), which are lacking virtually all HsMICOS components. (C, D, E) 2D STED nanoscopy of immunolabeled COS-7 (C), HeLa (D), and U-2 OS (E) cells expressing non-tagged DmMIC10b. Scale bars: 2 μm (A, B), 2 μm ((C, D, E), overview), and 0.5 μm ((C, D, E), inset).