Table 1.
List of oligonucleotides for PCR.
| Oligo | Oligo name | Sequence (5′-3′) | Internal |
|---|---|---|---|
| 1 | 28-5-Fw | CTTCGAAGCCAGTTTGCAAAAGGA | 7669 |
| 2 | 28-5-Rev | AAACTCCTTTTGCAAACTGGCTTC | 7670 |
| 3 | 28-3-Fw | CTTCGACTATTCGTTGTTAGTTTA | 7671 |
| 4 | 28-3-Rev | AAACTAAACTAACAACGAATAGTC | 7672 |
| 5 | 28-5′-arm Fw | GCAGGTGGAATTCTTGCATGCTAGCAAACATGATATAAGAGACCG | 7651 |
| 6 | 28-5′-arm Rev | CTCCTGTCATTCCTTTTGCAAACTGGCTTCTAC | 7652 |
| 7 | 28-5′-missing part Fw | CCAGTTTGCAAAAGGAATGACAGGAGGGCATGG | 7653 |
| 8 | 28-5′-missing part Rev | GGGCACTACGATCCTTTGTAAATTTCGATGTGCGTCAAG | 7654 |
| 9 | 28-attP-DsRed Fw | GAAATTTACAAAGGATCGTAGTGCCCCAACTGG | 7655 |
| 10 | 28-attP-DsRed Rev | AAATAAGTATATTTATAACTTCGTATAGCATACATTATACGAAGTTATACC | 7656 |
| 11 | 28-3′-missing arm Fw | ATACGAAGTTATAAATATACTTATTTAGTGCTTATTAATACTG | 7657 |
| 12 | 28-3′-missing arm Rev | ATTCGTTGTTAGTTTACAGGTTTATGGGTGATTTTTTTC | 7658 |
| 13 | 28-3′-arm Fw | CATAAACCTGTAAACTAACAACGAATAGTCAAAATG | 7659 |
| 14 | 28-3′-arm Rev | CTTGAACTCGATTGACGGAAGAGCCTTACAAAGGATGGACTGAGAAC | 7660 |
| 15 | EagI-DmMIC10b Fw | TTCGGCCGATGACTTCTCGCGATAATATTTTCG | 7794 |
| 16 | DmMIC10b-XhoI Rev | TTTACTCGAGTCATTTTAAAGAATTTAAATCCTTTTC | 8099 |
| 17 | DmMIC10b-FLAG-XhoI Rev | TTTACTCGAGTCACTTGTCGTCATCGTCTTTGTAGTCACCAGAGCCTCCTTTTAAAGAATTTAAATCCTTTTC | 7632 |
| 18 | Mic10b-G52/56L Fw | GCCTGTATGGCTCCTCGCTGGATTTTTAATGGGCATCGCTTATAG | 9580 |
| 19 | Mic10b-G52/56L Rev | CTATAAGCGATGCCCATTAAAAATCCAGCGAGGAGCCATACAGGC | 9581 |
| 20 | Mic10b-G54/58L Fw | GGCTCGGCGCTCTATTTGGAATGCTCATCGCTTATAGG | 9582 |
| 21 | Mic10b-G54/58L Rev | CCTATAAGCGATGAGCATTCCAAATAGAGCGCCGAGCC | 9583 |
| 22 | EagI-hMic10 Fw | TTCGGCCGATGTCTGAGTCGGAGCTC | 8237 |
| 23 | HMic10-FLAG Rev | TTTACTCGAGTCACTTGTCGTCATCGTCTTTGTAGTCACCAGAGCCTCCCTGCTCCTGCTCTTTGAC | 8226 |
| 24 | EagI-YMic10 Fw | TTCGGCCGATGTCCGAACAAGCACAAACAC | 8238 |
| 25 | Xho-YMic10-FLAG Rev | TTTACTCGAGTCACTTGTCGTCATCGTCTTTGTAGTCACCAGAGCCTCCAACCTTCGAGGATCTGAGGC | 8239 |
| 26 | DmMIC10b Fw | ATAAAGCTTGCCGCCACCATGACATCT | 10084 |
| 27 | DmMIC10b-FLAG Rev | AAACTCGAGTTACTTATCGTCATCGTCCTTATA | 10085 |
| 28 | DmMIC10b(C13S) Fw | GGAGAAGATCTCCAATCGCCTGG | 10175 |
| 29 | DmMIC10b(C13S) Rev | TCGAAGATGTTGTCCCTAG | 10176 |
| 30 | DmMIC10b(C19S) Fw | CCTGGACCACTCCGTGAGCGATG | 10177 |
| 31 | DmMIC10b(C19S) Rev | CGATTGCAGATCTTCTCCTCGAAG | 10178 |
| 32 | DmMIC10b(C28S) Fw | GATCAAGGGATCCGGAGGCGTGA | 10179 |
| 33 | DmMIC10b(C28S) Rev | AGCACATCGCTCACGCAG | 10180 |
| 34 | DmMIC10b(I41F) Fw | GTCCTTCCTGTTCCTGAAGAGGAG | 10183 |
| 35 | DmMIC10b(I41F) Rev | ACGGCGCTTCCGATGATC | 10184 |
| 36 | DmMIC10b(C64S) Fw | CTACCGGACCTCCGAGAAGGACC | 10181 |
| 37 | DmMIC10b(C64S) Rev | GCGATTCCCATTCCAAATC | 10182 |
| 38 | DmMIC10b(G29L) Fw | CAAGGGATGCCTGGGCGTGATCATC | 11737 |
| 39 | DmMIC10b(G29L) Rev | ATCAGCACATCGCTC | 11738 |
| 40 | DmMIC10b(G27/30L) Fw | GGACTGGTGATCATCGGAAGC | 11739 |
| 41 | DmMIC10b(G27/30L) Rev | GCACAGCTTGATCAGCACATCG | 11740 |
| 42 | DmMIC10b(G30/34L) Fw | TCATCCTGAGCGCCGTGTCCTTC | 11741 |
| 43 | DmMIC10b(G30/34L) Rev | TCACCAGTCCGCATCCCTTGATC | 11742 |
| 44 | DmMIC10b(hum. GX1) Fw | GTTTTGGATTAGGAAGCGCCGTGTC | 11743 |
| 45 | DmMIC10b(hum. GX1) Rev | CAGTACCTATCTTGATCAGCACATCGC | 11744 |
| 46 | TRE3G-DmMIC10b Fw | GCGCAAACTAGTATGTGTTCGATTCTAGATTCGAG | 9965 |
| 47 | TRE3G-DmMIC10b Rev | GCGCAAACCGGTCTTCAGGCTGTTCAGGTCCTTCTC | 9966 |
| 48 | EagI-ScMic10 Fw | GCGCAACGGCCGCCATGTCCGAACAAGCACAAACAC | 10068 |
| 49 | ScMic10-FLAG-XbaI Rev | GCGCAATCTAGATCACTACTTGTCGTCATCGTCTTTGTAGTCGCCTCCAACCTTCGAGGATCTGAGGCCAGCG | 10069 |
| 50 | Mic13_C90S Fw | CATGCTGCCCTCCTACGCAGGCA | 10250 |
| 51 | Mic13_C90S Rev | TGGATGAAACGGAAGGTGTTCTTCAC | 10251 |
| 52 | SalI-DmMic13 Fw | GCGCAAGTCGACATGGTTCTAGGATTTCTAGTGCGCG | 9921 |
| 53 | DmMic13-MluI Rev | GCGCAAACGCGTTTAGCTGCTCCAATGGCTCACGGCG | 9922 |
| 54 | SalI-HsMic13 Fw | GCGCAAGTCGACATGGTGGCCCGGGTGTGGTCGC | 10098 |
| 55 | HsMic13-MluI Rev | GCGCAAACGCGTCTAGCTGCTCCAATGGCTCACGGCGCGCACGCGGTCTGGCTTGGTGCGCGCCTTCACATACTCCC | 10099 |
| 56 | SalI-DmMic26 Fw | GCGCAAGTCGACATGCTGCGCAAAACGGCAACGATGG | 9925 |
| 57 | DmMic26-MluI Rev | GCGCAAACGCGTTTAGCTGCTCCAATGGCTCACGGCG | 9926 |
| 58 | SalI-DmMic19 Fw | GCGCAAGTCGACATGGGAGCCCGACAGTCTCAATCCC | 9923 |
| 59 | DmMic19-MluI Rev | GCGCAAACGCGTCTAGCTGCTCCAATGGCTCACGGCG | 9924 |
| 60 | CG41128 Fw | AAAGGCCATGGAAATGACTTCTCGCGATAATAT | 6337 |
| 61 | CG41128 Rev | GCGAATTCAAATTATTTTAAAGAATTTAAATCCTTTTCAC | 6338 |