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. 2023 Dec 7;31(1):150–158. doi: 10.1038/s41594-023-01124-2

Table 1.

Genetic modifications of R. capsulatus expression strain

Modification Deleted locus naturally encodes for Purpose for deletion Ref.
ΔnifHDK Molybdenum nitrogenase Remove the primary nitrogenase 38
ΔmodABC High-affinity molybdenum transporter Prevent molybdenum import to maximize expression levels of the alternative nitrogenase 35
ΔdraTG ADP-ribosyltransferase/ADP-ribosyl hydrolase system for the post-translational modification of nitrogenase iron proteins Ensure constitutive activity of the Fe-only nitrogenase 36
ΔgtaI Quorum sensing protein GtaI Removes the capsule to increase quality of cell pellet after centrifugation 37
ΔanfHDGK::gmR Fe-only nitrogenase Prevent genomic expression of wild-type Fe-only nitrogenase to enable recombinant expression of AnfHDGK variants from plasmid DNA 55