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. 2023 Dec 1;130(2):201–212. doi: 10.1038/s41416-023-02510-9

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© The Author(s), under exclusive licence to Springer Nature Limited 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 7 — a The images of multiple markers (DAPI, TCF1, CD11b, PD1, CD25, TIM3, CD3, CD4, CD8, and ac4C) were observed on the HCC microarray based on TSA IHC technology. b The images of ac4C+CD11b+ cells and CD3+CD8+PD1+ cells were shown in the I02 patient’s tissue. c The relative ac4c+CD11b+ cell ratio was calculated in both the tumour and adjacent tissues (p > 0.05). d The survival curve was created based on the HCC patients’ information (n = 70) and shows a better overall survival rate (p < 0.05) with high infiltration of ac4C+CD11b+ cells (n = 46). e The correlation analysis among ac4C+CD11b+ cells and T subsets was calculated. TSA tyramide signal amplification, IHC immunohistochemistry.