Fig. 3.

Suppression of ER stress attenuates mechanical overloading-induced ferroptosis by alleviating Ca²⁺ outflow from ER. A The qPCR of Bip, PERK, Calnexin, ACSL4 and GPX4 of the groups in this Figure (n = 3 for each group). B The Western blotting analysis for Bip, PERK, Calnexin, ACSL4, GPX4, Col-2 and MMP-13. C Quantification of Western blotting analysis (n = 3 for each group). D The Fura-2 AM to detect intracellular free Ca²⁺ level (n = 3 for each group). E–G DCFDA assays for ROS level and Mito-Tracker and JC-1 for mitochondrial membrane potential. Scale bar: 50 or 25 μm. H–I Quantitative analysis for DCFDA, Mito-Tracker, and JC-1 (n = 3 for each group). J The qPCR of Col-2 and MMP-13 of the groups in this Figure (n = 3 for each group). K Quantification of Western blotting analysis(n = 3 for each group). Results were shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001