Fig. 6.

Selenium supplementation alleviates the progression of IVDD in mice. A Flowchart of animal experiment on GPX4-CKO mice. B Flowchart of animal experiment on WT mice. C The concentration of selenium of NP tissues from mice (n = 3 for each group). D The MRI and Micro-CT for coccygeal discs of mice. E Quantitative analysis of MRI and Micro-CT (n = 3 for each group). F The Safranine O-Fast Green staining and Immunohistochemical assay of SelK, ATF6, GPX4, Col2 and MMP-13. Scale bars: 50 or 250 μm. G Quantitative analysis of the Safranine O-Fast Green staining and Immunohistochemical assay (n = 3 for each group). Results were shown as mean ± SEM. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001