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Relative expression of adaptive immunity genes: IgM (A), CD4 (B), β2M (C), and MHC class 2 (D) in the head kidney, liver, gills, and intestine of channel catfish fed with frass-supplemented diets (50–300 g/kg). Data are presented as mean ± SE, and multiple reference genes were used to normalize with the target gene (n = 4). Different alphabets indicate the significant differences between frass-supplemented diets (p < 0.05).
