Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 22;15:668. doi: 10.1038/s41467-024-44969-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Schematic of HYP-like cell fate induction by removing BRAF/MEK inhibitors. Created with BioRender.com. Strategies were indicated as follows: A, Spontaneous blastoids were formed by culturing in 5iLAF for 5 days; B, Spontaneous blastoids were formed by culturing in 5iLAF for 3 days first and then switched to 3iLAF (5iLAF-BRAFi-MEKi) for 2 days; C, Spontaneous blastoids were formed by culturing in 3iLAF for 5 days. b Spontaneous blastoid generation efficiency for the indicated conditions in a. n = 3 biological replicates; mean ± s.d.; Ordinary one-way ANOVA with Tukey’s multiple comparisons test; * means p < 0.05. c Representative immunofluorescence staining images of spontaneous blastoids containing HYP-like cells. Scale bars, 20 μm. d Comparison of embryonic datasets (left) with the spontaneous blastoids (right) after removing BRAF/MEK inhibitors from day 3 evidenced the HYP fate specification. PSA-EPI, primitive streak anlage stage epiblast. ExE extraembryonic, CTB cytotrophoblasts, STB syncytiotrophoblasts, EVT extravillous cytotrophoblasts. e Expression levels of blastocyst lineage markers for each cluster. f Enriched gene ontology (GO) terms in the differentially expressed genes of C5 compared against C0-3 identified using two-sided Wilcoxon Rank Sum Test with Bonferroni correction (Log2 Fold Change >0 and FDR < 0.05). The P values shown were computed with modified one-sided Fisher’s Exact test using David functional annotation tool. Source data are provided as a Source Data file.