TABLE 1.
Cytokine levels in supernatants of cocultures of Cryptococcus-laden monocytes plus T lymphocytes in the presence or absence of anti-GXM MAb (MAb 2H1)
| Stimulus(i) | Cytokine production (pg/ml)a
|
|
|---|---|---|
| IL-10 on day 3 | IL-2 on day 7 | |
| None | 2 ± 0 | 4 ± 0 |
| 7698 | 7 ± 1 | 29 ± 2 |
| 7698 + MAb 2H1b | 6 ± 1 | 28 ± 2 |
| 6995 | 42 ± 4 | 17 ± 1 |
| 6995 + MAb 2H1 | 3 ± 2c | 34 ± 2c |
a
IL-10 and IL-2 were determined in supernatant cocultures of monocytes laden with an encapsulated (6995) or acapsular (7698) strain of C. neoformans plus T lymphocytes. The results are the means of four separate experiments from four different donors ± SEMs.
b
MAb 2H1 (10 μg/ml) was added together with C. neoformans strains at the time of culture preparation.
c
P < 0.01 (MAb 2H1 plus C. neoformans [6995]-treated cells versus C. neoformans [6995]-treated cells) according to Student’s t test.