TABLE 3.

Proliferative response of T lymphocytes to monocytes laden with an encapsulated (6995) or acapsular (7698) strain of C. neoformans in the presence or absence of various doses of anti-GXM MAb (MAb 2H1)

Treatment	Response (cpm) for straina
	7968	6995
None	37,000 ± 3,000	21,000 ± 1,600
MAb 2H1b
0.1 μg/ml	38,000 ± 1,800	23,000 ± 3,000
0.25 μg/ml	34,000 ± 1,500	21,000 ± 3,600
5 μg/ml	39,000 ± 3,000	32,000 ± 1,900c
10 μg/ml	36,000 ± 5,000	36,000 ± 2,800c

^aProliferation of T lymphocytes was measured by [³H]thymidine incorporation after 7 days of incubation. The results are the means of four separate experiments from four different donors ± SEMs. 

^bMAb 2H1 was added together with a C. neoformans encapsulated (6995) or acapsular (7698) strain (2 × 10⁵) at the indicated doses to PBM (2 × 10⁴) for 2 h. Then, monocyte monolayers were washed and, subsequently, autologous T cells [T(E⁺), 10⁵] were added. 

^cP < 0.01 (MAb 2H1 plus C. neoformans [6995]-treated versus C. neoformans [6995]-treated cells) according to Student’s t test.