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. 2024 Jan 18;20:101–117. doi: 10.3762/bjoc.20.11

Table 2.

Gel-electrophoretic analysis of photoinduced DNA-strand cleavage in the presence of 3f depending on the irradiation time (A), on the concentration (B), and in the presence of 3c,eg (C). Lanes 1, 10 and 11 (A) and lane 11 (B): control experiment without 3f. Lanes 1 and 2 (B): control experiment without 3f, irradiated for 2 min. In all cases: c3 = 2.5 × 10−5 M, cDNA = 3.5 × 10−9 M, anaerobic conditions, irradiation time: 2 min, λmax = 366 nm.

A
supercoiled
open-circular
graphic file with name Beilstein_J_Org_Chem-20-101-i001.jpg

lane 1 2 3 4 5 6 7 8 9 10 11

irradiation time / min 0 0 0 0.5 1 2 5 10 15 15 15
Strand cleavage / % 11 9 9 16 25 38 73 83 73 24 26

B
graphic file with name Beilstein_J_Org_Chem-20-101-i002.jpg

lane 1 2 3 4 5 6 7 8 9 10 11

c / 10−5 M 0 0 1.3 1.3 2.5 2.5 3.8 3.8 5.0 5.0 0
strand cleavage / % 13 11 31 32 41 41 71 73 81 81 13

C
graphic file with name Beilstein_J_Org_Chem-20-101-i003.jpg

lane 1 2 3 4 5 6 7 8 9 10 11

irradiation time / min 0 2 2 0 2 0 2 0 2 0 2
substrate ref 3f 3f 3g 3g 3e 3e 3c 3c
strand cleavage / % 14 15 31 15 42 15 46 16 51 17 39