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. 2024 Jan 23;17:2. doi: 10.1186/s13072-024-00526-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Sin3B^−/− LSKs display defective stress hematopoiesis. A LSKs were sorted from Sin3B^F/F and Sin3B^H−/− mice 9 days after 5-FU administration (100 mg/kg, i.p.) and subjected to scRNA-Seq analysis utilizing the same strategy as in Fig. 1. n = 2 mice per genotype. B Sin3B^+/+ and Sin3B^−/− cells were able to be separated in our data based on hashtag oligonucleotide sequencing. C Quantification of different hematopoietic subsets at indicated conditions. D Monocle3 differentiation trajectory analysis of Sin3B^+/+ and Sin3B^−/− LSKs during a stress response. E Pseudotime analysis utilizing LT-HSCs as starting node for either Sin3B^+/+ and Sin3B^−/− LSKs. F Differential expression analysis was used to calculate modules of genes grouped together based on expression as a function of pseudotime. G Gene Ontology analysis for 100 genes in common. H Gene Ontology analysis for 136 genes unique to Sin3B^+/+ LT-HSCs. i Gene Ontology analysis for 126 genes unique to Sin3B^−/− LT-HSCs