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. Author manuscript; available in PMC: 2024 Jan 23.
Published in final edited form as: Biochemistry. 2023 Mar 27;62(8):1376–1387. doi: 10.1021/acs.biochem.2c00686

Fig. 2.

Fig. 2.

A short RNA duplex bearing 8-azaN binds tightly to ADAR1. (A) 16 bp duplex derived from yeast HER1 RNA. X is A in native target sequence but is varied (8-azaN, A, or G) in the succeeding experiments. (B) Gel shifts of ADAR1d with H16 8-azaN or A at 0, 2, 4, 8, 10, 20, 40, 80, 100, 150, 200, 300 nM ADAR1d E1008Q and 5 nM H16 RNA. (C) Fitted plots of fraction H16 RNA bound vs protein concentration. Data was plotted to the equation: y = A*[x/(KD+x)] where y = fraction H16 RNA bound, x = [ADAR1d]; A = binding endpoint; and KD = dissociation constant. Error bars represent standard deviation from n = 3 technical replicates.